Difference between revisions of "Part:BBa K2066030"

Revision as of 23:35, 19 October 2016

pTAC sfGFP

This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship without RiboJ.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 132

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 <partinfo>BBa_K2066030 short</partinfo>
-pTAC Ribozyme Characterization Part – GFP only; no RiboJ
+This part is used for 2016 WM iGEM Ribozyme Characterization project. Lou et. al showed that sequences in the 5' UTR of a gene can affect the input-output relationship (transfer function) of a circuit. The RiboJ serves as an insulator to generalize the transfer function of a circuit regardless of promoter. The part is used in combination with BBa_K2066016 (constitutive Lac Repressor) to look at input-output relationship without RiboJ.
-This part was designed to be assembled via Gibson Assembly from WM16_014 (using primers WM16_P027 and WM16_P009) and the same WM16_014 again (using primers WM16_P019 and WM16_P025).
-This part follows Lou et al. 2012 (“Ribozyme-based insulator parts buffer synthetic circuits from genetic context”). This part will be used in concert with WM16_031 (pTAC Ribozyme Chracterization Part – cI GFP; no RiboJ) to determine the influence of the RiboJ insulator sequence on gene expression.
 <!-- Add more about the biology of this part here