Difference between revisions of "Part:BBa K2150015:Design"

 
(Source)
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===Source===
 
===Source===
 
PTet,RBS,TetX,GFP,double terminator all come from the kit.
 
  
 
===References===
 
===References===

Revision as of 10:39, 12 October 2016


pTet GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

The fusion protein can accurately report the expression level of TetX with fluorescence intensity of GFP. PTet is used to test the expression of tetX at the presence of tetracycline conveniently.


Source

References