Difference between revisions of "Part:BBa K1962016"

Revision as of 21:46, 22 October 2016

A device for expression of Im-E3 in response to pH

This is a composite part for the regulated expression of the Immunity Protein for netralization of the Colicin E3, which is a specific RNase. The composite part was assembled from a pH responsive promoter (asr BBa_K1231000), an RBS (BBa_B0030) and the biobrick encoding the Immunity Protein against E3 (Im-E3) (BBa_K1962004).

Usage and Biology

We cloned the E3-Immunity protein (BBa_K1962016) downstream of a pH sensitive promoter Pasr (BBa_K1231000) and a bile salt sensitive promoter PacrRA (BBa_K1231001) to generate the composite parts (BBa_K1962016) and (BBa_K1962012), respectively.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 This is a composite part for the regulated expression of the Immunity Protein for netralization of the Colicin E3, which is a specific RNase. The composite part was assembled from a pH responsive promoter (<i>asr</i> <partinfo>BBa_K1231000</partinfo>), an RBS (<partinfo>BBa_B0030</partinfo>) and the biobrick encoding the Immunity Protein against E3 (Im-E3) (<partinfo>BBa_K1962004</partinfo>).
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 ===Usage and Biology===
+We cloned the E3-Immunity protein (<partinfo>BBa_K1962016</partinfo>) downstream of a pH sensitive promoter Pasr (<partinfo>BBa_K1231000</partinfo>) and a bile salt sensitive promoter PacrRA (<partinfo>BBa_K1231001</partinfo>) to generate the composite parts (<partinfo>BBa_K1962016</partinfo>) and (<partinfo>BBa_K1962012</partinfo>), respectively.
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