Difference between revisions of "Part:BBa K1884001"

Revision as of 08:53, 12 October 2016

Gal4BD-CIB1

Cryptochrome-interacting basic-helix-loop-helix 1(CIB1) is a protein-coded gene. The product of this gene expression is a basic helix-loop-helix (bHLH) protein which would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. This part is a Gal4 DNA binding domain fused to N terminus of CIB1

Usage and Biology

This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA binding domian fused to its N terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. CRY2 will disconnected with CIB1 in the dark and halt the DNA transcription. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 218
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 637
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 137

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 ===Usage and Biology===
-This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA bindin domian fused to its N terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor.
+This fusion protein is for use in a yeast-two-hybrid system,and a Gal4 DNA binding domian fused to its N terminus. In order to control DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein brings together two halves of a split transcription factor. CRY2 will disconnected with CIB1 in the dark and halt the DNA transcription.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===