Difference between revisions of "Part:BBa K1949030:Design"
(→Source) |
|||
| Line 15: | Line 15: | ||
===Source=== | ===Source=== | ||
| − | The gene sequence was isolated from E. coli BL21 | + | The gene sequence was isolated from E. coli BL21<br> |
| + | |||
| + | ===Materials and Methods=== | ||
| + | |||
| + | =====Construction===== | ||
| + | |||
| + | =====Assay protocol===== | ||
| + | |||
| + | |||
| + | |||
| + | |||
===References=== | ===References=== | ||
Revision as of 02:16, 16 October 2016
yafO
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 335
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
yafO gene fragment amplified by PCR contains XbaI site at N-terminal and SpeI and PstI site at C-terminal. Both terminals were cut by respective restriction enzymes and the fragment used for ligation with iGEM plasmid vectors.
fwd:5’-AAATCTAGATGCGGGTATTCAAAACAAAACTTA-3’
rev:5’-AAACTGCAGCGGCCGCTACTAGTATTATTAAAAACGCATGCGAAACGCTTCTGCCA-3’
Source
The gene sequence was isolated from E. coli BL21
Materials and Methods
Construction
Assay protocol
===References===