Difference between revisions of "Part:BBa K1887000"
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The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside). | The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside). | ||
Revision as of 04:01, 12 October 2016
LacZ
The lacZ gene from Lactobacillus acidophilus was introduced in the His locus of L. lactis NZ9000 genome by temperature sensitive vector, which makes the NZ-Blue strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
References
Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.