Difference between revisions of "Part:BBa K1949050:Design"
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===References===
 
===References===
 +
Ochiai S, Yasumoto S, Morohoshi T, Ikeda T. AmiE, a Novel <i>N</i>-Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate <i>Acinetobacter</i> sp. Strain Ooi24. 2014 Nov;80(22):6919-25.

Revision as of 19:12, 11 October 2016


amiE


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A point mutation is introduced to amiE to remove the EcoRI recognition site; the 291th nucleotide was changed from A to a G.


Source

The DNA fragment of amiE was artificially synthesized.


References

Ochiai S, Yasumoto S, Morohoshi T, Ikeda T. AmiE, a Novel N-Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24. 2014 Nov;80(22):6919-25.