Difference between revisions of "Part:BBa K1926011"
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This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.  
 
This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.  
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1926011 SequenceAndFeatures</partinfo>
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===Usage===
  
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===You may use this part to:===
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You may use this part to:
  
 
1) Localize proteins in vivo with BLOCK and dye from NEB company;
 
1) Localize proteins in vivo with BLOCK and dye from NEB company;
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===Source:===
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===Source===
  
 
The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.
 
The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.
  
  
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===Construction Design===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K1926011 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 08:58, 11 October 2016

The SNAP UNIT: SNAP-tag flanked by loxP

This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 419
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

You may use this part to:

1) Localize proteins in vivo with BLOCK and dye from NEB company;

2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus.


Source

The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.


Construction Design