Difference between revisions of "Part:BBa K1897005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. | + | HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the ''Serratia marcescens''. HasI is a componentof the system and this cytoplasmic protein is usually bound to the anti-sigma factor [https://parts.igem.org/Part:BBa_K1897003 HasS] and therefore is inactive. When the [https://parts.igem.org/Part:BBa_K1897001 holo-HasA hemophore] binds to the cell surface receptor [https://parts.igem.org/Part:BBa_K1897002 HasR], it triggers a conformational change in [https://parts.igem.org/Part:BBa_K1897003 HasS]. This then releases the HasI which will then trigger expression of genes under the control of the [https://parts.igem.org/Part:BBa_K1897006 Has promoter]. |
Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007]) | Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007]) | ||
Latest revision as of 21:13, 10 October 2016
HasI sigma factor coding sequence
Usage and Biology
HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the Serratia marcescens. HasI is a componentof the system and this cytoplasmic protein is usually bound to the anti-sigma factor HasS and therefore is inactive. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which will then trigger expression of genes under the control of the Has promoter.
Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)
References
Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 42
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]