Difference between revisions of "Part:BBa K1897003"

Revision as of 19:03, 10 October 2016

HasS antisigma factor coding sequence

Usage and Biology

HasS (34.7 kDa) is the anti-sigma factor in the Has system signalling cascade. The Has system is a heme acquisition system originally from the Serratia marcescens. HasS is responsible for binding to the cytoplasmic extracellular sigma factor, HasI, inhibiting its activity. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which binds to the Has promoter. This will allow for the expression of genes controlled by that promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 34
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 317
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 72
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 Characterisation of this part is done in tandem with the full has operon ([https://parts.igem.org/Part:BBa_K1897007 BBa_K1897007])
+===References===
+Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). ''Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition.'' Molecular microbiology, 53(4), 1267-1277.
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 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K1897003 SequenceAndFeatures</partinfo>