Difference between revisions of "Part:BBa K2114020:Design"
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===Source=== | ===Source=== | ||
| − | The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). | + | The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare). |
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Revision as of 01:36, 20 October 2016
CgeA_G4S_HA_GST
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1129
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 267
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 526
Design Notes
Introduction of HA epitope tag and flexible GGGGS linker by primer extensions and PCR.
Source
The cgeA gene comes from the genome of B. subtilis. The glutathione S-transferase gene was amplified from the pGEX-6P-1 vector (GE healthcare).