Difference between revisions of "Part:BBa K1933002"
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<partinfo>BBa_K1933002 short</partinfo>
 
<partinfo>BBa_K1933002 short</partinfo>
  
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This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. This coding sequence was fused to INPNC-His (part number: ) or BclA-His (part number: ) in our project.
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This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1]. <br>This coding sequence was fused to INPNC-His ([https://parts.igem.org/Part:BBa_K1933001 BBa_K1933001]) or BclA-His ([https://parts.igem.org/Part:BBa_K1933000 BBa_K1933000]) in our project.
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For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]
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<br>For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]
  
  
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[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology 86.4 (2014): 558-567.
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[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." '' Journal of medical virology, '' 86.4, (2014): 558-567.

Revision as of 08:48, 11 October 2016


anti-Norovirus GII.4 scFv

This part codes for anti-Norovirus GII.4 scFv (single-chain variable fragment) which derives from human 12A2 antibody. This scFv is suggested to prevent binding of human norovirus to Hist-Blood Group Antigens[1].
This coding sequence was fused to INPNC-His (BBa_K1933001) or BclA-His (BBa_K1933000) in our project.
For more information, please visit [http://2016.igem.org/Team:Kyoto our wiki]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage

Biology

Characterization

Reference

Judging

[1]Higo‐Moriguchi, Kyoko, et al. "Isolation of cross‐reactive human monoclonal antibodies that prevent binding of human noroviruses to histo‐blood group antigens." Journal of medical virology, 86.4, (2014): 558-567.