Difference between revisions of "Part:BBa K2088010"
 
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<partinfo>BBa_K2088010 short</partinfo>
 
<partinfo>BBa_K2088010 short</partinfo>
  
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Before the  XylE - catechol 2,3-dioxygenase, the promoter J23100 and the RBS B0034 are connected. The device can be used to degrade the catechol in some degradation system to decrease the content of the catechol for the catechol can restrain the growth of the bacteria, also, the catechol is a kind of common degradation intermediate product.
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In this device, promoter J23100 and the RBS B0034 are connected before the XylE - catechol 2,3-dioxygenase.  
  
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====Verification Test====
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Catechol is one of the main metabolites during the degradation of aromatic compounds. This device is useful to degrade catechol in some degradation systems to reduce the toxicity of catechol to cells. With the strong promoter and rbs, xylE can convert to 2-hydroxymuconate semialdehyde, a bright yellow product which can be metabolized by E.coli.
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*We verified the function of xylE by constructing a device [https://parts.igem.org/Part:BBa_K2088010 BBa_K2088010].
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[[Image:XylE_device_(BBa_K2088010)_before_dripping_catechol.jpg|thumb|500px|center|Figure 8: We construced a device that includes J23100, B0034, xylE and B0014. Transform the device into E.coli and culture at 37℃ overnight, the colonies showed a common white colour.]]
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[[Image:XylE_device_(BBa_K2088010)_after_dripping_catechol.jpg|thumb|500px|center|Figure 9: Drip 0.2mol/L catechol onto the colonies, the cells became bright yellow after 10 minutes. This indicates the xylE converts catechol to 2-hydroxy-cis,cis-muconic semialdehyde. ]]
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<partinfo>BBa_K2088010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2088010 SequenceAndFeatures</partinfo>
  
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====Qualitative Experiment====
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*We verified the function of xylE by constructing a device [https://parts.igem.org/Part:BBa_K2088010 BBa_K2088010].
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[[Image:XylE_device_(BBa_K2088010)_before_dripping_catechol.jpg|thumb|500px|center|Figure 8: We construced a device that includes J23100, B0034, xylE and B0014. Transform the device into E.coli and culture at 37℃ overnight, the colonies showed a common white colour.]]
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[[Image:XylE_device_(BBa_K2088010)_after_dripping_catechol.jpg|thumb|500px|center|Figure 9: Drip 0.2mol/L catechol onto the colonies, the cells became bright yellow after 10 minutes. This indicates the xylE converts catechol to 2-hydroxy-cis,cis-muconic semialdehyde. ]]
  
 
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Latest revision as of 18:39, 18 October 2016


A device that expresses the XylE- catechol 2,3-dioxygenase to degrade the catechol .

In this device, promoter J23100 and the RBS B0034 are connected before the XylE - catechol 2,3-dioxygenase.

Catechol is one of the main metabolites during the degradation of aromatic compounds. This device is useful to degrade catechol in some degradation systems to reduce the toxicity of catechol to cells. With the strong promoter and rbs, xylE can convert to 2-hydroxymuconate semialdehyde, a bright yellow product which can be metabolized by E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 572
    Illegal AgeI site found at 923
  • 1000
    COMPATIBLE WITH RFC[1000]

Qualitative Experiment

  • We verified the function of xylE by constructing a device BBa_K2088010.
Figure 8: We construced a device that includes J23100, B0034, xylE and B0014. Transform the device into E.coli and culture at 37℃ overnight, the colonies showed a common white colour.
Figure 9: Drip 0.2mol/L catechol onto the colonies, the cells became bright yellow after 10 minutes. This indicates the xylE converts catechol to 2-hydroxy-cis,cis-muconic semialdehyde.