Difference between revisions of "Part:BBa K2100003"
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===Usage and Biology===
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<img src="https://static.igem.org/mediawiki/2016/thumb/4/4f/T--MIT--hEF1aeYFP_Attractene_Optimization.jpg/240px-T--MIT--KHB1177menstrual-cycle.jpeg" alt = 'Transfection Optimization Data for hEF1a-eYFP' style='width: 250px: height = 250px; float:right;"  margin: 0 1.5%;>
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Revision as of 17:10, 10 October 2016


pENTR hEF1a

Enter description about hef1a promoter here. The pENTR hEF1a part is an entry vector containing the human elongation factor 1 alpha (hEF1a) promoter, acting as a constituitive mammalian promoter.

We have characterized the hEF1a promoter in all four cell lines we experimented on in the duration of our project using the following fluorescent tags: eYFP, mKate, and (tag?)BFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal BglII site found at 591
    Illegal BamHI site found at 1197
    Illegal XhoI site found at 990
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 1210
    Illegal PstI site found at 337
    Illegal PstI site found at 842
    Illegal NgoMIV site found at 725
    Illegal AgeI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]