Difference between revisions of "Part:BBa K2042019:Design"
RATOVOMANANA (Talk | contribs) (→Source) |
RATOVOMANANA (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This sequence was designed as a spacer to prevent errors in transcription by placing the RBS close to the promoter. | + | This sequence was designed as a spacer to prevent errors in transcription by placing the RBS close to the promoter. Formation of a hairpin structure or self complementary sequences was avoided. |
===Source=== | ===Source=== | ||
Latest revision as of 20:54, 12 October 2016
Spacer in between promoter and coding sequence
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was designed as a spacer to prevent errors in transcription by placing the RBS close to the promoter. Formation of a hairpin structure or self complementary sequences was avoided.
Source
Synthetic synthesis