Difference between revisions of "Part:BBa K1898250"

 
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<partinfo>BBa_K1898250 short</partinfo>
 
<partinfo>BBa_K1898250 short</partinfo>
  
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BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The ORF includes GSR, or glutathione reductase that catalyses the conversion of glutathione disulfide to glutathione, and 10x Histidine tag for protein purification. BBa_B0015 consists of a double terminator to end transcription.  
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BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The construct codes for glutathione reductase (GSR) and 10x Histidine tag. GSR is a catalyst for the conversion of glutathione disulfide to glutathione and 10x Histidine tag is used for protein purification. BBa_B0015 consists of a double terminator to end transcription.  
  
 
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<partinfo>BBa_K1898250 parameters</partinfo>
 
<partinfo>BBa_K1898250 parameters</partinfo>
 
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==Gel Pictures==
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PCR was set up after the construct was cloned. The expected PCR band sizes are ~2.1kb, which is shown in lane __:
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==Sequencing==
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The DNA was sent to sequencing. The sequencing result is correct and shown below:

Revision as of 06:59, 16 October 2016


Strong promoter + Strong RBS + GSR + 10x Histidine tag + Double terminator

BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The construct codes for glutathione reductase (GSR) and 10x Histidine tag. GSR is a catalyst for the conversion of glutathione disulfide to glutathione and 10x Histidine tag is used for protein purification. BBa_B0015 consists of a double terminator to end transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 804
    Illegal BamHI site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1199
    Illegal SapI.rc site found at 1607


Gel Pictures

PCR was set up after the construct was cloned. The expected PCR band sizes are ~2.1kb, which is shown in lane __:

Sequencing

The DNA was sent to sequencing. The sequencing result is correct and shown below: