Difference between revisions of "Part:BBa K1898100:Design"
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===Design Notes===
 
===Design Notes===
 
We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech.  
 
We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech.  
 +
Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.
  
  

Revision as of 14:47, 10 October 2016


ch25h, cholesterol 25 hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.


Source

cDNA of ch25h was ordered from Sino BIological Inc

References