Difference between revisions of "Part:BBa K1926021"

 
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This part is the SNAP-VIKA UNIT of cyclebow including a SNAP UNIT and a VIKA UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The VIKA UNIT, which can be cut off by VIKA, includes an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.
  
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You may use this part to:
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1) Test the cut-off efficiency of recombinase CRE and VIKA by qRT-PCR;
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2) Estimate the time needed for genome repair because VIKA can only be expressed after the cut off of SNAP UNIT and genome repair;
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3) Fast cut off this part by transient transfecting recombinase gene into nucleus;
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4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.

Revision as of 05:03, 10 October 2016

This part is the SNAP-VIKA UNIT of cyclebow including a SNAP UNIT and a VIKA UNIT. The SNAP UNIT, which can be cut off by CRE, includes an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The VIKA UNIT, which can be cut off by VIKA, includes an sv40 nuclear location sequence (NLS), a recombinase gene named VIKA and an sv40 terminator flanked by homodromous recognition site of recombinase VIKA named Vox.


You may use this part to:

1) Test the cut-off efficiency of recombinase CRE and VIKA by qRT-PCR;

2) Estimate the time needed for genome repair because VIKA can only be expressed after the cut off of SNAP UNIT and genome repair;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus;

4) When there is no CRE, you can use this part as the SNAP UNIT, which can help localize proteins in vivo with BLOCK and dye from NEB company.