Difference between revisions of "Part:BBa K1926011"

 
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This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.
  
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===You may use this part to:===
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1) Localize proteins in vivo with BLOCK and dye from NEB company;
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2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;
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3) Fast cut off this part by transient transfecting recombinase gene into nucleus.
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===Source:===
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The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.

Revision as of 05:57, 11 October 2016

This part is the SNAP UNIT of cyclebow system including an sv40 nuclear location sequence (NLS), a covalent labeling of fusion proteins named SNAP-tag® and an sv40 terminator flanked by homodromous recognition site of recombinase CRE named loxP. The whole part can be cut off by CRE.


You may use this part to:

1) Localize proteins in vivo with BLOCK and dye from NEB company;

2) Dye after blocking, you are able to see whether an event have an influence on the localization and expression of the interested protein;

3) Fast cut off this part by transient transfecting recombinase gene into nucleus.


Source:

The SNAP-tag® was brought from NEB company. NLS and sv40 terminator were got from commercial plasmid: pentry nls GFP/pcdna3.1.