Difference between revisions of "Part:BBa K1926001"
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<partinfo>BBa_K1926001 short</partinfo>
 
<partinfo>BBa_K1926001 short</partinfo>
  
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This part is a cyclic promoter named Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]
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This part is a cyclic promoter of protein Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]
  
 
1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.
 
1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.
  
 
2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.
 
2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.
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You may use this part to:
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 +
1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line;
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2) Use it as a human promoter by transient transfecting it into cells.
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Source:
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The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:
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pCCNE-F: CGTGTTTACATTCCACCCGCGCCA
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pCCNE-R: TGATGGGGCTGCTCCGGCCT
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(Product: 986)
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:47, 11 October 2016


A cyclic promoter of Cyclin E from human genome

This part is a cyclic promoter of protein Cyclin E from human genome. It can lead to transcription of the downstream DNA sequence once in every G1 phase[1,2]

1. Hwang, H.C. and B.E. Clurman, Cyclin E in normal and neoplastic cell cycles. Oncogene, 2005. 24(17): p. 2776-86.

2. Ohtani, K., J. DeGregori, and J.R. Nevins, Regulation of the cyclin E gene by transcription factor E2F1. Proc Natl Acad Sci U S A, 1995. 92(26): p. 12146-50.


You may use this part to:

1) Express something in mammal cell lines particularly in G1 phases or once in every cell cycle by stable transfecting it into cell line;

2) Use it as a human promoter by transient transfecting it into cells.


Source:

The sequence was retrieved from Addgene. We got it from human genome through PCR using the following primers:

pCCNE-F: CGTGTTTACATTCCACCCGCGCCA

pCCNE-R: TGATGGGGCTGCTCCGGCCT

(Product: 986)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 382
    Illegal NotI site found at 497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 529
    Illegal XhoI site found at 1050
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 273
    Illegal NgoMIV site found at 313
    Illegal NgoMIV site found at 491
  • 1000
    COMPATIBLE WITH RFC[1000]