Difference between revisions of "Part:BBa K2148002"
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This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick. | This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations. | ||
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<partinfo>BBa_K2148002 parameters</partinfo> | <partinfo>BBa_K2148002 parameters</partinfo> | ||
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+ | ===Characterization=== | ||
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+ | ===References=== | ||
+ | Bateman J. M., Purton S. (2000). Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Mol. Gen. Genet. 263, 404–410. 10.1007/s004380051184 |
Revision as of 23:17, 9 October 2016
aphA6
This is the coding DNA sequence that confers antibiotic resistance to amikacin, neomycin, and kanamycin. The CDS is flanked by Phytobrick fusion sites which corresponds to a CDS/CTAG Phytobrick.
Usage and Biology
This part can be used with other level 0 Phytobrick to create a transcriptional unit to provide a selection pressure on transformations.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 647
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 647
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 647
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 647
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 647
- 1000COMPATIBLE WITH RFC[1000]
Characterization
References
Bateman J. M., Purton S. (2000). Tools for chloroplast transformation in Chlamydomonas: expression vectors and a new dominant selectable marker. Mol. Gen. Genet. 263, 404–410. 10.1007/s004380051184