Difference between revisions of "Part:BBa K2092001"

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alcR, ethanol-activated transcription factor from A. nidulans

This part encodes for AlcR protein, which is a transcriptional factor. It binds to alcA promoter and aldA promoter to induce expression of proteins in the presence of ethanol or threonine.

Usage and Biology

alcR is a positive regulatory gene in the ethanol regulon of filamentous fungus Aspergillus nidulans (A.nidulans). It encodes a protein that acts as a transcription factor which would bind to its target promoters alcA [1] and aldA. The expression of the downstream gene of alcA is strongly induced through the positive transcriptional regulator AlcR protein by various substrates such as ethanol and threonine.

AlcR is also known as a zinc binuclear cluster activator as it contains a DNA-binding domain belonging to the C6 zinc binuclear cluster family. AlcR is unique in that it can bind to symmetric and asymmetric DNA sites with the same apparent affinity and also bind to single site with high affinity [2].

References

[1] Panozzo, C., Capuano, V., Fillinger, S. and Felenbok, B. (1997). The zinc binuclear cluster Activator AlcR is able to bind to single sites but requires multiple repeated sites for synergistic activation of the alcA gene in Aspergillus nidulans. Journal of Biological Chemistry, 272(36), 22859–22865.

[2] Felenbok, B., Sequeval, D., Mathieu, M., Sibley, S., Gwynne, D.I. and Davies, R.W. (1988). The ethanol regulon in Aspergillus nidulans: Characterization and sequence of the positive regulatory gene alcR. Gene, 73(2), 385–396.

Sequence and Features