Difference between revisions of "Part:BBa K1968001:Design"
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
The part was ordered as gblock through IDT and was subsequently cloned into PhytoBricks Universal Acceptor plasmids (BBa_P10500).  
+
The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.  
  
 
===References===
 
===References===
 +
(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500

Revision as of 19:02, 9 October 2016


Synechocystis Pcpc560 Phytobrick promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

Nucleotide G at position 109 was mutated to a A in order to remove a BbsI site, which would have interfered with later MoClo assemblies.


Source

The sequence of this part was outlined in (1). This part was ordered as a gBlock Gene Fragment from IDT and was assembled into the PhytoBricks Universal Acceptor (BBa_P10500). The sequence of this part was confirmed by Sanger Sequencing.

References

(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500