Difference between revisions of "Part:BBa K2008007:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Flanking | + | Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Flanking <i>amyE</i> regions of homology were added to allow for chromosomal integration and increased long-term stability of the <i>comK</i> construct. |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | This part was amplified from the pAX01-comK plasmid constructed by Zhang and Zhang, 2010, and was previously characterized by the UofC_Calgary 2014 iGEM team. Flanking | + | This part was amplified from the pAX01-comK plasmid constructed by Zhang and Zhang, 2010, and was previously characterized by the UofC_Calgary 2014 iGEM team. Flanking <i>amyE</i> regions of homology were derived from the <i>B. subtilis</i> 168 genome. |
===References=== | ===References=== | ||
Latest revision as of 19:39, 18 October 2016
Xylose->comK with AmyE homology
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 322
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 862
Design Notes
Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Flanking amyE regions of homology were added to allow for chromosomal integration and increased long-term stability of the comK construct.
Source
This part was amplified from the pAX01-comK plasmid constructed by Zhang and Zhang, 2010, and was previously characterized by the UofC_Calgary 2014 iGEM team. Flanking amyE regions of homology were derived from the B. subtilis 168 genome.