Difference between revisions of "Part:BBa K1968001"
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<partinfo>BBa_K1968001 short</partinfo> | <partinfo>BBa_K1968001 short</partinfo> | ||
| − | Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. | + | Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. Pcpc560 contains two predicted promoters and 14 predicted transcription factor binding sites (1). |
(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500 | (1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500 | ||
Revision as of 19:11, 9 October 2016
Synechocystis Pcpc560 Phytobrick promoter
Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. Pcpc560 contains two predicted promoters and 14 predicted transcription factor binding sites (1).
(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2