Difference between revisions of "Part:BBa K2074031"
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<partinfo>BBa_K2074031 short</partinfo> | <partinfo>BBa_K2074031 short</partinfo> | ||
− | + | Cry11Aa and Cyt1 are protein from Bacillus thuringiensis (Bt) which have toxicity to the larvae of mosquito.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito. | |
+ | [[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry4Aa and Cyt2 Toxins Group]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 08:27, 18 October 2016
Cry11Aa(Codon optimization)+Extended FMDV 2A+Cyt1(Codon optimization)
Cry11Aa and Cyt1 are protein from Bacillus thuringiensis (Bt) which have toxicity to the larvae of mosquito.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquito.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 517
Illegal NgoMIV site found at 1681
Illegal NgoMIV site found at 2029 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 49
Illegal BsaI.rc site found at 244
Illegal BsaI.rc site found at 1459
Illegal BsaI.rc site found at 2548
Illegal SapI.rc site found at 1440