Difference between revisions of "Part:BBa K2074028"

Revision as of 08:18, 18 October 2016

Cry4Aa(Codon optimization)+Extended FMDV 2A+Cyt2(Codon optimization)

Cry4Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt.) which have toxicity to the larvae of mosquitoes.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the toxicity to the larvae of mosquitoes.

Fig.1 Cry4Aa+2A+Cyt1 Toxins Express Device

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 986
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1378
Illegal NgoMIV site found at 3631
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 31
Illegal BsaI.rc site found at 184
Illegal BsaI.rc site found at 2071
Illegal BsaI.rc site found at 2611
Illegal BsaI.rc site found at 2920
Illegal BsaI.rc site found at 3487
Illegal BsaI.rc site found at 4150
Illegal SapI.rc site found at 1188
Illegal SapI.rc site found at 1230

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2074028 short</partinfo>
-cry4Aa and cyt2 are protein from Bacillus thuringiensis (or Bt),which have toxicity to the larve of mosquito.we use 2A system to express two protein at the same time in Chlamydomonas reinhardti.These two protein express at the same time can enhance the  toxicity to the larve of mosquito.
+Cry4Aa and Cyt2 are proteins from Bacillus thuringiensis (Bt.) which have toxicity to the larvae of mosquitoes.We used 2A system to express two proteins at the same time in Chlamydomonas reinhardti. These two proteins express at the same time can enhance the  toxicity to the larvae of mosquitoes.
+[[File:2016FAFUCHINADevice5.png|800px|thumb|center|'''Fig.1''' Cry4Aa+2A+Cyt1 Toxins Express Device ]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===