Difference between revisions of "Part:BBa K1963006"

Latest revision as of 12:20, 23 October 2016

A translational fusion between E. coli OsmY and Serratia Hfq

The E. coli OsmY protein (BBa_K892008) is secreted from the cell, and this has been characterised by at least two iGEM teams and other scientists in the field (e.g. BBa_K1963007). Serratia Hfq is an RNA binding protein (see BBa_K1963001). This translational fusion between the two may allow Hfq to be secreted from an E. coli chassis organism, together with any specific sRNA that may be attached. Pairing Serratia Hfq and Serratia sRNA sequences may afford some specificity to the system.

Usage and Biology

This biobrick was used as the basis of a rhamonose-regulated composite part BBa_K1963009.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1963006 short</partinfo>
-The E. coli OsmY protein is secreted from the cell. Serratia Hfq is an RNA binding protein. This translational fusion between the two may allow Hfq to be secreted from an E. coli chassis organism, together with any specific sRNA that may be attached. Pairing Serratia Hfq and Serratia sRNA sequences may afford some specificity to the system.
+The <i>E. coli</i> OsmY protein (<partinfo>BBa_K892008</partinfo>) is secreted from the cell, and this has been characterised by at least two iGEM teams and other scientists in the field (e.g. <partinfo>BBa_K1963007</partinfo>). <i>Serratia</i> Hfq is an RNA binding protein (see <partinfo>BBa_K1963001</partinfo>). This translational fusion between the two may allow Hfq to be secreted from an <i>E. coli</i> chassis organism, together with any specific sRNA that may be attached. Pairing <i>Serratia</i> Hfq and <i>Serratia</i> sRNA sequences may afford some specificity to the system.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+This biobrick was used as the basis of a rhamonose-regulated composite part <partinfo>BBa_K1963009</partinfo>.
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