Difference between revisions of "Part:BBa K2074026:Experience"

 
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<partinfo>BBa_I0500 AddReview 5</partinfo>
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<I><b>[https://2019.igem.org/Team:FAFU-CHINA]</B></I>
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[https://2019.igem.org/Team:FAFU-CHINA] The Hsp70A-RbcS2 promoter enhances the expression of EGFP after heat shock initiation. It is seen that the FAFU-CHINA team in 2016 made Western blost on the expression of EGFP, at a temperature of 40 degrees Celsius. Heat shock 0, 80, 60, 40, 20min.
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This year (2019), we used the same promoter and EGFP for QPCR quantification, provided that the temperature was 40 ° C, heat shock 0, 40, 80, 100, 120, 140 min. The specific data we have obtained is the result of the following tables and images:
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[[Image:https://static.igem.org/mediawiki/parts/8/86/HeatShockTime.png|left|thumb|400px|''Heat Shock Time(min).''']]
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[[Image:https://static.igem.org/mediawiki/parts/9/90/T--FAFU-CHINA--EGFP.png|left|thumb|400px|'''EGFP characterization data.''']]
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The results showed that after 0, 40, 80 min, the results were the same as the 2016 FAFU-CHINA results. The new supplement condition we have this year is that the heat shock is 100, 120, 140min. The experimental results show that the relative expression of EGFP is increasing under these conditions, but the results are similar at 120 and 140 min, this may be the limit of the conditions that enhance EGFP expression.
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Revision as of 02:03, 22 October 2019


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2074026

User Reviews

UNIQ3befd7ea9a4c2819-partinfo-00000000-QINU

•••••

[1]

[2] The Hsp70A-RbcS2 promoter enhances the expression of EGFP after heat shock initiation. It is seen that the FAFU-CHINA team in 2016 made Western blost on the expression of EGFP, at a temperature of 40 degrees Celsius. Heat shock 0, 80, 60, 40, 20min. This year (2019), we used the same promoter and EGFP for QPCR quantification, provided that the temperature was 40 ° C, heat shock 0, 40, 80, 100, 120, 140 min. The specific data we have obtained is the result of the following tables and images:


File:Https://static.igem.org/mediawiki/parts/8/86/HeatShockTime.png
Heat Shock Time(min).'
File:Https://static.igem.org/mediawiki/parts/9/90/T--FAFU-CHINA--EGFP.png
EGFP characterization data.

The results showed that after 0, 40, 80 min, the results were the same as the 2016 FAFU-CHINA results. The new supplement condition we have this year is that the heat shock is 100, 120, 140min. The experimental results show that the relative expression of EGFP is increasing under these conditions, but the results are similar at 120 and 140 min, this may be the limit of the conditions that enhance EGFP expression.

UNIQ3befd7ea9a4c2819-partinfo-00000002-QINU