Difference between revisions of "Part:BBa K2074021"

 
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our part is cry4Aa+2A which have toxicity to the larve of mosquito.We transferred the gene ofcry4Aa+2 into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
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https://static.igem.org/mediawiki/igem.org/thumb/9/9f/T--FAFU-CHINA--partsclones.png/800px-T--FAFU-CHINA--partsclones.png<br />
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Our part is cry4Aa+2A which have toxicity to the larve of mosquito.We transferred the gene ofcry4Aa+2 into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:31, 18 October 2016


Cry4Aa(Codon optimization) - Extended FMDV


800px-T--FAFU-CHINA--partsclones.png

Our part is cry4Aa+2A which have toxicity to the larve of mosquito.We transferred the gene ofcry4Aa+2 into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 986
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1378
    Illegal NgoMIV site found at 3631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 31
    Illegal BsaI.rc site found at 184
    Illegal BsaI.rc site found at 2071
    Illegal BsaI.rc site found at 2611
    Illegal BsaI.rc site found at 2920
    Illegal BsaI.rc site found at 3487
    Illegal SapI.rc site found at 1188
    Illegal SapI.rc site found at 1230