Difference between revisions of "Part:BBa K2070004:Experience"

 
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how you used this part and how it worked out.
 
how you used this part and how it worked out.
  
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===Applications of BBa_K2070004===
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<partinfo>BBa_K2070004 </partinfo>
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<I>iGEM UT-Tokyo 2016</I>
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Our Experimental results show that the part work as expected, with the GFP expression higher in presence of ECF sigma35 expression.
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[[File:T--UT-Tokyo--BBa K2070005-1.png|thumb|700px| '''Measurement of ECF sigma35 and sigma30 promoter activity.''' Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state. ]]
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[[File:T--UT-Tokyo--BBa K2070005-2.png|thumb|700px| '''Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. '''Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard  deviation.
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===User Reviews===
 
===User Reviews===

Revision as of 12:49, 20 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

BBa_K2070004 iGEM UT-Tokyo 2016

Our Experimental results show that the part work as expected, with the GFP expression higher in presence of ECF sigma35 expression.

Measurement of ECF sigma35 and sigma30 promoter activity. Fluorescence/OD600 of a construct containing both an ECF sigma35 generating device and ECF sigma 35 inducible GFP generating device BBa_K2070005 was compared to that containing only either one of the devices. *** for P> 0.001 indicates an extremely significant difference between the induced and uninduced state.
Measurement of Activated Sigma30 Promoter Activity in Comparison to Promoter BBa_J23101. Both parts are ligated in plasmid pSB3K3. The part containing the constitutive sigma35 generator was double transformed into the cell for measurement. Data represents an average of 3 samples and error bars represent the standard deviation.
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User Reviews

UNIQ9e426540c0967b6e-partinfo-00000001-QINU UNIQ9e426540c0967b6e-partinfo-00000002-QINU