Difference between revisions of "Part:BBa K1887000:Design"

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===References===
 
===References===
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Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.

Revision as of 08:35, 14 October 2016


LacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lacZ gene from Lactobacillus acidophilus was introduced into the His locus of L. Lactis genome by temperature sensitive vector, which makes the knocked-in strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).

Source

Using the genomic DNA of Lactobacillus acidophilus as the template, the lacZ gene product is obtained by PCR and cloned into the pSB1C3 plasmid.

References

Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.