Difference between revisions of "Part:BBa K1957004"

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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1957004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1957004 SequenceAndFeatures</partinfo>
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[[File:T--NRP-UEA-Norwich--Sequencing_Data_6072_1_.jpg|frame|none|Figure 2: Sequencing data aligned with HydA gene insert using the software Benchling. (A) Screenshot of sequencing data at 28bp (B) Screenshot of sequencing data at 528bp]]
  
  

Revision as of 13:58, 9 October 2016


HydA subunit of FeFe Hydrogenase

One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.

Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 6 and 7 have HydA (607) gene insert, which is 1325bp. Ladder is Hyperladder Kb, sizes shown in bp

DNA of the colony on lane 6 and 7 were sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.

Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1261
    Illegal SapI site found at 1219
File:T--NRP-UEA-Norwich--Sequencing Data 6072 1 .jpg
Figure 2: Sequencing data aligned with HydA gene insert using the software Benchling. (A) Screenshot of sequencing data at 28bp (B) Screenshot of sequencing data at 528bp