Difference between revisions of "Part:BBa K2027012:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
TBA
+
Part BBa_K274001 was codon-optimized and we took into the considerations from Gosset (https://www.ncbi.nlm.nih.gov/pubmed/24225202) in order to create a feedback-resistant version of the tyrosinase in order to overproduce melanin. The part was synthesized by IDT with tails to 2-part Gibson Assembly into pSB1C3.
  
  
 +
[[File:MutMelA_SV.png|500px|thumb|left|Sequence Verification]]
  
−
===Source===
 
  
−
TBA
+
 
 +
===Source===
 +
https://www.ncbi.nlm.nih.gov/pubmed/24225202
 +
http://partsregistry.org/Part:BBa_K274001
  
 
===References===
 
===References===

Revision as of 15:11, 27 October 2016


Mutant Feedback-Resistant Version of MelA Tyrosinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1379
    Illegal AgeI site found at 1661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1231
    Illegal BsaI.rc site found at 25
    Illegal BsaI.rc site found at 536


Design Notes

Part BBa_K274001 was codon-optimized and we took into the considerations from Gosset (https://www.ncbi.nlm.nih.gov/pubmed/24225202) in order to create a feedback-resistant version of the tyrosinase in order to overproduce melanin. The part was synthesized by IDT with tails to 2-part Gibson Assembly into pSB1C3.


File:MutMelA SV.png
Sequence Verification


Source

https://www.ncbi.nlm.nih.gov/pubmed/24225202 http://partsregistry.org/Part:BBa_K274001

References