Difference between revisions of "Part:BBa K2027012"

 
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<partinfo>BBa_K2027012 short</partinfo>
 
<partinfo>BBa_K2027012 short</partinfo>
  
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Mutant Feedback-Resistant Version of MelA Tyrosinase
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Mutant Feedback-Resistant Version of MelA Tyrosinase. The MutmelA gene endogenous to Rhizobium etli encodes a feedback resistant tyrosinase that has been shown to be effective when transfected into E. coli. [8] Based on the work of MI Chávez-Béjar, our team sought to increase the production of L-tyrosine in E. coli through metabolic engineering to shuttle the flow of carbon from the cells’ central metabolism into the shikimate pathway.
  
 
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Revision as of 15:08, 27 October 2016


Mutant Feedback-Resistant Version of MelA Tyrosinase

Mutant Feedback-Resistant Version of MelA Tyrosinase. The MutmelA gene endogenous to Rhizobium etli encodes a feedback resistant tyrosinase that has been shown to be effective when transfected into E. coli. [8] Based on the work of MI Chávez-Béjar, our team sought to increase the production of L-tyrosine in E. coli through metabolic engineering to shuttle the flow of carbon from the cells’ central metabolism into the shikimate pathway.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1379
    Illegal AgeI site found at 1661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1231
    Illegal BsaI.rc site found at 25
    Illegal BsaI.rc site found at 536