Difference between revisions of "Part:BBa K2151001"

Revision as of 20:50, 8 October 2016

p25, moderate constitutive S. thermophilus promoter

This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the Streptococcus thermophilus genome, alongside weaker promoters p8 and p20.

We used p25 as a candidate promoter to drive gene expression in both Streptococcus thermophilus and E. coli. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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   <p>This part consists of p25, a medium constitutive bacterial promoter, contained within pSB1C3. Its intended use is to promote transcription of an inserted gene. p25 was initially identified in a promoter probe survey of the <i>Streptococcus thermophilus</i> genome, alongside weaker promoters p8 and p20.</p>
-  <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101 130% as strong as J23106).</p>
+  <p>We used p25 as a candidate promoter to drive gene expression in both <i>Streptococcus thermophilus</i> and <i>E. coli</i>. Quantification of p25 using GFP revealed promoter strength on par with medium Anderson promoters (30.8% as strong as J23100, 43.7% as strong as J23101, 130% as strong as J23106).</p>
 <!-- Add more about the biology of this part here