Difference between revisions of "Part:BBa K1957004"

Revision as of 21:43, 8 October 2016

HydA subunit of FeFe Hydrogenase

One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.

Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 6 and 7 have HydA (607) gene insert, which is 1325bp. Ladder is Hyperladder Kb, sizes shown in bp

DNA of the colony on lane 6 and 7 were sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.

Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1261
Illegal SapI site found at 1219

@@ Line 5: / Line 5: @@
 One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically this is the HydA subunit which is the membrane bound catalytic subunit. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
-[[File:T--NRP-UEA-Norwich--Gel_PCR_Colony_FeFe_.jpg|thumb|Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 6 and 7 have HydA (607) gene insert, which is 1325bp. Ladder is Hyperladder Kb, sizes shown in bp]]
+[[File:T--NRP-UEA-Norwich--Gel_PCR_Colony_FeFe_.jpg|thumb|none|Figure 1: PCR Colony of FeFe Hydrogenase subunits. Lanes 6 and 7 have HydA (607) gene insert, which is 1325bp. Ladder is Hyperladder Kb, sizes shown in bp]]
 DNA of the colony on lane 6 and 7 were sent off for sequencing. After sequencing confirmation it was used for the Golden Gate cloning and submitted into the iGEM registry.