Difference between revisions of "Part:BBa K2142004"
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The sequence encodes for the dam methylase. This metylase is normally important for methylating DNA after replication. In our project we over expressed the translation of the protein which leads to an mutation increase by the factor of 50. The part is no standard BioBrick and can be found in BBa_K2142005 | The sequence encodes for the dam methylase. This metylase is normally important for methylating DNA after replication. In our project we over expressed the translation of the protein which leads to an mutation increase by the factor of 50. The part is no standard BioBrick and can be found in BBa_K2142005 | ||
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| − | + | ===Information contributed by City of London UK (2021)=== | |
| − | + | Part information is collated here to help future users of the BioBrick registry. | |
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| + | dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [https://pubmed.ncbi.nlm.nih.gov/368070] | ||
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| + | It is likely to act in postreplication mismatch repair [https://pubmed.ncbi.nlm.nih.gov/6308634] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [https://pubmed.ncbi.nlm.nih.gov/25845600] | ||
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| + | Furthermore, it may also be involved in DNA replication [https://pubmed.ncbi.nlm.nih.gov/3891329] | ||
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| + | ====Reaction==== | ||
| + | a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine | ||
| + | |||
| + | [https://www.rhea-db.org/rhea/15197] | ||
Revision as of 10:26, 26 July 2021
Dam methylase
The sequence encodes for the dam methylase. This metylase is normally important for methylating DNA after replication. In our project we over expressed the translation of the protein which leads to an mutation increase by the factor of 50. The part is no standard BioBrick and can be found in BBa_K2142005
Information contributed by City of London UK (2021)
Part information is collated here to help future users of the BioBrick registry.
dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]
It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]
Furthermore, it may also be involved in DNA replication [4]
Reaction
a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine