Difference between revisions of "Part:BBa K1884001:Design"
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===Source=== | ===Source=== | ||
| − | + | We gain this part from Shenzhen Engineering Laboratory of Marine Algal Biotechnology. | |
===References=== | ===References=== | ||
Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391. | Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391. | ||
Latest revision as of 22:34, 19 October 2016
Gal4BD-CIB1
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 218
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 637
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 137
Design Notes
Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1. They interact in a blue light-specific manner in yeast and Arabidopsis cells, acting together with additional CIB1-related proteins to promote CRY2-dependent floral initiation. The modules require no exogenous chromophore, trigger protein interactions on a subsecond time scale that are reversible within minutes and can be activated by two-photon microscopy, allowing potential use in vivo in whole organisms.
Source
We gain this part from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.
References
Hughes R M, Bolger S, Tapadia H, et al. Light-mediated control of DNA transcription in yeast[J]. Methods, 2012, 58(4): 385-391.