Difference between revisions of "Part:BBa K2114001:Design"
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | Amplification of cotZ from genomic DNA of Bacillus subtilis. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS) | + | Amplification of cotZ from genomic DNA of <i>Bacillus subtilis</i>. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS) |
===References=== | ===References=== | ||
Latest revision as of 06:33, 20 October 2016
aGFPnano_HA_aHelix_cotZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 465
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Introduction of HA epitope tag and alpha helical linker by primer extensions.
Source
Amplification of cotZ from genomic DNA of Bacillus subtilis. The anti-GFP nanobody was provided by Dr. Maximilian Ulbrich (BIOSS)