Difference between revisions of "Part:BBa K2060002:Experience"
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===Applications of BBa_K2060002=== | ===Applications of BBa_K2060002=== | ||
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| + | <b>Early Characterisation</b> | ||
| + | <p>We had limited success in characterization of this biobrick. Even though the part sequence is correct and we were able to show a blue light output, we did not demonstrate the hoped for red light output.</p> | ||
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| + | <img width=100% src="https://static.igem.org/mediawiki/parts/a/ac/T--Cardiff_Wales--BBa_K2060002_ImageA.png"> | ||
| + | </div> | ||
| + | </html> | ||
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| + | <p>However we do not rule out the usage of this biobrick in the future. Our evidence shows that LUXoperon is robustly expressed but that maintenance of mKeima expression is non-trivial. We also found this with our attempts to express mKeima as an individual protein in the biobrick [https://parts.igem.org/Part:BBa_K1135001 BBa_K1135001]. | ||
| + | <p>Therefore an exciting challenge for future iGEM teams will be to trouble shoot the expression of the entire [https://parts.igem.org/Part:BBa_K2060002 LUXoperon-mKeima] biobrick. The potential red light output will be a valuable tool in the use of bioluminescent bacteria in synthetic biology. | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 16:56, 14 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2060002
Early Characterisation
We had limited success in characterization of this biobrick. Even though the part sequence is correct and we were able to show a blue light output, we did not demonstrate the hoped for red light output.
However we do not rule out the usage of this biobrick in the future. Our evidence shows that LUXoperon is robustly expressed but that maintenance of mKeima expression is non-trivial. We also found this with our attempts to express mKeima as an individual protein in the biobrick BBa_K1135001. <p>Therefore an exciting challenge for future iGEM teams will be to trouble shoot the expression of the entire LUXoperon-mKeima biobrick. The potential red light output will be a valuable tool in the use of bioluminescent bacteria in synthetic biology.
User Reviews
UNIQ82859caf4f608fe4-partinfo-00000001-QINU
UNIQ82859caf4f608fe4-partinfo-00000002-QINU