Difference between revisions of "Part:BBa K2060002:Design"

 
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===Source===
 
===Source===
  
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rbs-mKeima sequence was synthesised and added to BBa_K325909 by Gibson assembly.
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rbs-mKeima sequence was synthesised and added to BBa_K325909 by NEB HiFi assembly.
  
 
===References===
 
===References===

Latest revision as of 11:21, 13 October 2016


LUXoperon with mKeima


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 4171
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3169
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5558
    Illegal BsaI.rc site found at 2567
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 5883


Design Notes

We used a longer RBS sequence upstream of mKeima to enable co-translation of mKeima along with the components of the LUXoperon


Source

rbs-mKeima sequence was synthesised and added to BBa_K325909 by NEB HiFi assembly.

References