Difference between revisions of "Part:BBa K1957003"

 
m
Line 5: Line 5:
 
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically, this is the HydB subunit which is the central subunit. This basic part has a strep-tag attached to its N-terminus for consequent protein assays. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
 
One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically, this is the HydB subunit which is the central subunit. This basic part has a strep-tag attached to its N-terminus for consequent protein assays. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.
  
 +
Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:36, 8 October 2016


HydB (N-terminally tagged) subunit of FeFe Hydrogenase

One of the three subunits that make up FeFe Hydrogenase in Shewanella oneidensis. Specifically, this is the HydB subunit which is the central subunit. This basic part has a strep-tag attached to its N-terminus for consequent protein assays. This unit is one out of the three subunits that can be used to make a the entire FeFe hydrogenase construct.

Restriction site incompatibility, [1000], is with regards to MoClo i.e. Golden Gate cloning. As we used this in downstream cloning experiments the restriction site could not be avoided.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 369