Difference between revisions of "Part:BBa K2110014"
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
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<partinfo>BBa_K2110014 SequenceAndFeatures</partinfo>
  

Latest revision as of 17:08, 13 October 2016


MHETase enzyme after codon optimization for bacillus subtilis

This gene encodes the MHETase enzyme. This protein can bind on the PET surface and degrade MHET to TPA and EG, which have higher efficiency than other cutinase. This enzyme cooperates with PETase enzyme well in PET degradation. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimization for bacillus subtilis for better expression and secretion.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
    Illegal NgoMIV site found at 387
    Illegal AgeI site found at 1086
  • 1000
    COMPATIBLE WITH RFC[1000]