Difference between revisions of "Part:BBa K2110013"
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<partinfo>BBa_K2110012 short</partinfo>
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<partinfo>BBa_K2110013 short</partinfo>
  
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This promoter is a enhanced promoter used in Saccharomyces cerevisiae. It regulates the 3-phosphoglycerate kinase (PGK1) gene in Saccharomyces cerevisiae. This promoter can be used in both plasmid and genome of Saccharomyces cerevisiae.
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This gene encodes thePETase enzyme. This protein can bind on thePET surface and degrade PET to MHET, which have higher efficiency than other cutinase. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimizationfor bacillus subtilis for better expression and secretion.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2110012 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2110013 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2110012 parameters</partinfo>
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<partinfo>BBa_K2110013 parameters</partinfo>
 
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Revision as of 08:01, 6 October 2016


PETase enzyme after codon optimization for bacillus subtilis

This gene encodes thePETase enzyme. This protein can bind on thePET surface and degrade PET to MHET, which have higher efficiency than other cutinase. At first, this gene couldn’t fully express in bacillus subtilis. So we use codon optimizationfor bacillus subtilis for better expression and secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 639
  • 1000
    COMPATIBLE WITH RFC[1000]