Difference between revisions of "Part:BBa K2020001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]). <br> | ||
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT. Moreover, the leader sequence pelB begins with a start codon and was placed in front of the subtilisin E gene to generate a functional coding sequence that can be expressed in E. coli. | The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT. Moreover, the leader sequence pelB begins with a start codon and was placed in front of the subtilisin E gene to generate a functional coding sequence that can be expressed in E. coli. | ||
Revision as of 20:06, 8 October 2016
subtilisin E gene with leader sequence pelB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000).
The sequence of the subtilisin E gene from Bacillus subtilis was codon optimized for E. coli with the DNA and protein sequence analysis software "Geneious" and additionally with the "Codon Optimization Tool" from IDT. Moreover, the leader sequence pelB begins with a start codon and was placed in front of the subtilisin E gene to generate a functional coding sequence that can be expressed in E. coli.
Source
The sequence of the signal peptide pelB was obtained through the Registry (BBa_J32015). Together with the subtilisin E gene (BBa_K2020000) it was ordered from IDT.