Difference between revisions of "Part:BBa K1969004"

 
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__NOTOC__
 
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{|width='80%'
 
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|width=35% valign='top'|
 
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[[Image:Terminator.png]]
 
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<partinfo>BBa_K1969004 parameters</partinfo>
 
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<partinfo>BBa_K1969004 short</partinfo>
 
<partinfo>BBa_K1969004 short</partinfo>
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* Transcription terminator for alcohol dehydrogenase 1.
 
  
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This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression.
  
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'''Secondary Structure'''
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<!-- Add more about the biology of this part here
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===Usage and Biology===
  
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[[Image:Mfold-K1969004-1.png]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1969004 SequenceAndFeatures</partinfo>
  
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<hr>
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'''Measurement'''
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<!-- Uncomment this to enable Functional Parameter display
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* [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
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===Functional Parameters===
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<partinfo>BBa_K1969004 parameters</partinfo>
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Revision as of 14:53, 8 October 2016


GAL1 promoter

This is the full length of yeast GAL1 promoter cloned from genomic DNA of Saccharomyces cerevisiae. In the range of 1-118 bp of the promoter, there is an upstream activating sequence mediating GAL4-dependent induction. All the galactose structural genes (GAL1, GAL10, GAL7, GAL2) are coordinately regulated at the level of transcription in response to galactose by Gal4p, Gal80p, and Gal3p. Regardless of carbon source, the Gal4p transcriptional activator is bound as a dimer to upstream activation sites found in the promoters of the GAL genes. In the presence of galactose, Gal3p sequesters the transcriptional repressor Gal80p in the cytoplasm, thereby relieving inhibition of Gal4p and resulting in GAL gene expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]