Difference between revisions of "Part:BBa K2020001"
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<partinfo>BBa_K2020001 short</partinfo> | <partinfo>BBa_K2020001 short</partinfo> | ||
| − | This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000). It can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins. | + | This composite part consists of the leader sequence pelB ([[Part:BBa_J32015|BBa_J32015]]) and the subtilisin E gene ([[Part:BBa_K2020000|BBa_K2020000]]). It can be used to express subtilisin E in ''Escherichia coli'' and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins. |
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Revision as of 17:05, 5 October 2016
subtilisin E gene with leader sequence pelB
This composite part consists of the leader sequence pelB (BBa_J32015) and the subtilisin E gene (BBa_K2020000). It can be used to express subtilisin E in Escherichia coli and simultaneously secret the enzyme into the periplasm of the cell. Subtilisin E is an alkaline serine protease which non-specifically digests proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 54
- 1000COMPATIBLE WITH RFC[1000]