Difference between revisions of "Part:BBa K2036024"
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When body intake large amount of lactose .The lactose signal as pulse signal induce the transcription of downstream components. Then cro, Cll and Clll will have a low and transient expression level , these parts form a positive feedback system and direct stable expression of Cro, Cro binds to cro binding site and blocks the pRM promoter so that only gene iLDH will be expressed and can transform lactic acid into pyruvate.<br> | When body intake large amount of lactose .The lactose signal as pulse signal induce the transcription of downstream components. Then cro, Cll and Clll will have a low and transient expression level , these parts form a positive feedback system and direct stable expression of Cro, Cro binds to cro binding site and blocks the pRM promoter so that only gene iLDH will be expressed and can transform lactic acid into pyruvate.<br> | ||
When PH is in the range of 7~9 , Cl will be expressed and bind to Cl binding site ,then block the expression of iLDH nd Clll, which will shut down positive feedback and lead to the expression of β-galactosidase and the degradation of lactose.<br> | When PH is in the range of 7~9 , Cl will be expressed and bind to Cl binding site ,then block the expression of iLDH nd Clll, which will shut down positive feedback and lead to the expression of β-galactosidase and the degradation of lactose.<br> | ||
| − | + | [[File:T--HUST-China--Appication-Fig2-circuit.png|thumb|300px|center|Whole circuit of the application of prokaryotic signal filter.]] | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 09:39, 3 October 2016
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
Whole circuit of the application of our prokaryotic signal filter.
Main function:
We construct this circuit to apply our prokaryotic signal filter.
As the figure shows, Clll(BBa_K2036001) can combine with Ftsh and thus competitively preventing the degradation of Cll and Cll can activate the promoter pRE.
When body intake large amount of lactose .The lactose signal as pulse signal induce the transcription of downstream components. Then cro, Cll and Clll will have a low and transient expression level , these parts form a positive feedback system and direct stable expression of Cro, Cro binds to cro binding site and blocks the pRM promoter so that only gene iLDH will be expressed and can transform lactic acid into pyruvate.
When PH is in the range of 7~9 , Cl will be expressed and bind to Cl binding site ,then block the expression of iLDH nd Clll, which will shut down positive feedback and lead to the expression of β-galactosidase and the degradation of lactose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3212
Illegal BamHI site found at 3251
Illegal BamHI site found at 3943 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]