Difference between revisions of "Part:BBa K2036030"
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<partinfo>BBa_K2036030 short</partinfo> | <partinfo>BBa_K2036030 short</partinfo> | ||
| − | ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit. | + | ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit.([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki]) |
[[File:T--HUST-China--Description-Fig-prokaryote.png|thumb|400px|center|Fig1:bi-stable function of Signal Filter Eukaryotic circuit]] | [[File:T--HUST-China--Description-Fig-prokaryote.png|thumb|400px|center|Fig1:bi-stable function of Signal Filter Eukaryotic circuit]] | ||
Revision as of 09:21, 3 October 2016
PP2CA-TTADH1-pCyc-Kozak-Snrk2.2-TTADH1-pCyc-Kozak-ABF2-TTADH1-prd29A-Kozak-GFP-LVAssrAtag
ABF2 can be phosphorylated by Snrk2.2 to activate promoter RD29A and thus starting the expression of the target gene and also forming a positive feedback system. ABF2 can be dephosphorylated by PP2CA to stop the whole process, making the bi-stable state of our circuit.([http://2016.igem.org/Team:HUST-China Details see to HUST-China 2016 wiki])
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 3756
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 3756
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 90
Illegal BglII site found at 3824
Illegal BamHI site found at 661
Illegal BamHI site found at 806
Illegal BamHI site found at 918
Illegal BamHI site found at 1816
Illegal BamHI site found at 2473
Illegal XhoI site found at 65
Illegal XhoI site found at 875 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 3756
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 3756
Illegal AgeI site found at 48 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5268
Illegal BsaI.rc site found at 5939